Microbial biomarkers as an indicator of ecosystem recovery following surface mine reclamation

Increased disturbance of terrestrial ecosystems in recent years for purposes of mineral extraction has created interest in development and optimization of reclamation methodologies for these lands. Currently, criteria for judging surface mine reclamation success, or progress toward reclamation goals, predominantly rely on aboveground indicators that fail to account for the abundance and composition of soil microbiota, an essential aspect of soil health. To test the utility of fatty acid methyl ester (FAME) biomarkers as indicators of reclamation progress, FAME bacterial, fungal, and total biomass biomarkers extracted from soil of surface mine reclamation sites of different ages and an adjacent undisturbed site were compared with other indicators of reclamation progress and ecosystem stability. Our results indicate that FAME microbial biomarkers and soil organic matter (SOM) contents were greatly impacted by disturbance. Discriminant analysis of FAME bacterial, fungal and total microbial biomass biomarkers, although clearly able to discriminate between disturbed and undisturbed ecosystems, indicated a trend towards the undisturbed condition with reclamation age. The ratio of FAME bacterial to fungal biomarkers reflected changes in other indicators of soil health (SOM, inorganic N concentration), suggesting that this ratio is a useful indicator of reclamation progress. © 2002 Published by Elsevier Science B.V.